Objective: To explore the implementation points, application value, and impact of evidence-based nursing on patients’ postoperative pain after laparoscopic surgery for gallbladder stones. Methods: According to the guidance of the random number table method, patients with gallbladder stones who underwent laparoscopic surgery in our hospital from January 2025 to December 2025 were divided into a control group and an experimental group, with a total of 86 cases in the two groups; among them, routine nursing was carried out in the control group, and evidence-based nursing was carried out in the experimental group. 43 cases were included in each group to compare the final effects. Results: (1) After laparoscopic surgery for patients with gallbladder stones, the experimental group had shorter recovery time and hospitalization time than the control group, P < 0.05; (2) In terms of VAS score, there was no difference between the groups among patients with gallstones before nursing, P > 0.05; after nursing intervention, the experimental group (1.98 ± 0.74 points) was lower, P < 0.05; (3) The incidence of complications after laparoscopic surgery was 18.60% for patients with gallbladder stones in the control group and 4.65% in the experimental group. The comparison results showed that the experimental group was lower, P < 0.05; (4) In terms of nursing satisfaction, the patients with gallbladder stones (97.67%) in the experimental group were at a higher level, P < 0.05. Conclusion: After laparoscopic surgical treatment, the implementation of evidence-based nursing is of great significance. For patients with gallbladder stones, it can significantly shorten the recovery time, reduce the patient’s pain symptoms, and ensure satisfaction. More importantly, this nursing can reduce the incidence of patient complications, has a high safety factor, and is worthy of further promotion.