Volume 10,Issue 4
Q-Markers of Paris Polyphylla var. yunnanensis Based on Hemostatic Efficacy
To enhance the quality control of traditional Chinese medicine (TCM) and ensure clinical medication safety and efficacy, this study developed a quality assessment method for Paris polyphylla var. yunnanensis that was aligned with its hemostatic efficacy. The methodology involved a multi-tiered screening strategy that used plasma recalcification time (PRT) and prothrombin time (PT) as pharmacodynamic endpoints to systematically evaluate the crude herb, bioactive fractions, and purified compounds. Based on these evaluations, the weights of the core criteria (efficacy, measurability, specificity) were determined using the Analytic Hierarchy Process (AHP), supported by objective data quantification from the Entropy Weight Method (EWM). The results showed that all compounds except water-soluble fraction A significantly shortened PRT and PT (P < 0.001). Eleven active constituents were identified, with polyphyllin VII and H exhibiting the most significant activity. Consequently, the AHP-EWM model prioritized five saponins as core Q-markers based on their integrated hemostatic efficacy contributions, ranked as follows: Paris saponin I > Paris saponin II > Paris saponin VII > Paris saponin H > Paris saponin D. This study thereby provides a scientific basis for improving the quality control of Paris polyphylla var. yunnanensis and its formulations by elucidating its multi-component hemostatic mechanism and quantitatively identifying five core Q-markers.
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