Volume 10,Issue 4
Performance Evaluation of Autologous Thrombin Produced from Platelet‑rich Plasma (PRP) Tubes
Thrombin derived from bovine sources is commonly used to arrest bleeding during surgical procedures. However, there are risks associated with the use of bovine-derived thrombin, such as postoperative bleeding and the risk of infection in patients. Therefore, it is essential to develop a technology to generate autologous thrombin. In this study, autologous thrombin was produced from platelet-poor plasma (PPP) obtained using PRP tubes, mixed with 10% calcium gluconate, and halloysite nanotubes (HNTs), and evaluated the stability of prepared thrombin when stored at room temperature. The experiment demonstrated that the combination of 3 ml of platelet-poor plasma (PPP), 2.3 ml of 10% calcium gluconate, and a minimum of 1.5 mg of HNTs produces autologous thrombin with enhanced activity and stability, providing experimental evidence for its potential clinical application.
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