Volume 11,Issue 1
Determination of the Content of Hydroxyl Safflor Yellow A in Mongolian Medicine Yihahari-12
Objective: To establish a method for determining the content of Mongolian medicine Yihahari-12 by establishing a high-performance liquid chromatography analysis method. Method: High-performance liquid chromatography was used to determine the content of hydroxysafflor yellow pigment A in Ihrhari-12. The specific experimental conditions were as follows: a chromatographic column with an inner diameter of 4.6 mm, a length of 250 mm, and a particle size of 5 μm was used as a filler, and octadecylsilane bonded silica gel was used; The column temperature is 35℃; The mobile phase is methanol acetonitrile (ratio of 26:74); The detection wavelength is set to 403 nm, the flow rate is 1 mL/min, and the injection volume is 20 μL. The linear relationship investigation shows that hydroxysaffron yellow pigment A exhibits a good linear relationship within the concentration range of 0.02 mg/mL to 0.10 mg/mL. The regression equation is Y=-0.000631+0.0x, with an R value of 0.9999; The average recovery rate is 93.66%. As a result, this content determination method for hydroxysaffron yellow pigment A has the characteristics of good stability, good repeatability, strong specificity, and convenience and speed.
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