Volume 10,Issue 3
Determination of Genistin Content in Flemingia philippinensis Merr. et Rolfe by High Performance Liquid Chromatography (HPLC)
To set up the HPLC method in determination of the genistin content in Flemingia philippinensis Merr. et Rolfe. Method: Extract and filter the crushed Flemingia philippinensis Merr. et Rolfe that has been soaked in methanol, the filtrate of which is to be taken out as the sample. Shimadzu LC-2010A is adopted in this experiment which is performed on Agilent Extend-RP column with acetonitrile-0.1% phosphoric acid aqueous solution (19:81) as mobile phase. The flow rate is 1.0 mL/min and the UV detective wavelength is 262 nm. Result: The linear relationship is excellent with higher recovery rate within the range. This method is rapid, simple and accurate.
1. National Administration of Traditional Chinese Medicine "Chinese Materia Medica" Editorial Board. "Chinese Materia Medica." Volume 4, Shanghai: Shanghai Science and Technology Press, 1999:476.
2. Meng QR. Flemingia: Effects of Jianshen Zhuang waist. Medicine and health care. 1998,3:52.
3. Chinese Pharmcopocia Commission. Chinese Pharmacopoeia(2005), Beijing: Chemical and Industrial Press, 2005(Appendix III):21.
4. Hunan Provincial Department of Health. Hunan Chinese herbal standards. Hunan: Hunan Science and Technology Press, 1993:34.
5. The Guangdong Provincial Food and Drug Administration made. Guangdong Provincial Traditional Chinese Medicine criteria. Volume I. Guangzhou: Guangdong Science and Technology Press, 2004,11:17.
6. Shanghai Municipal Health Bureau. Shanghai Traditional Chinese Medicine criteria. Shanghai: Shanghai Science and Technology Press, 1994:26.
7. Health Department of Guangxi Zhuang Autonomous Region. Guangxi standard Chinese herbal medicines Volume II. Guangxi: Guangxi Science and Technology Press, 1996,10:37.
8. Zhang LX, Peng JM, Ma J. Journal of Chinese Medicinals,2007,30 (7):887-890.
9. Tang YP, Lou FCH, Ma W et al.. Journal of China Pharmaceutical University,2001,32 (3):187-189.
10. Wen Ma, Weihui Wang, Yan Peng, Qiaoxia Bian, Nannan Wang, David Y.-W. Lee, Ronghua Dai. Ultra-high performance liquid chromatography with tandem mass spectrometry method for the simultaneous quantitation of five phthalides in rat plasma: Application to a comparative pharmacokinetic study of Huo Luo Xiao Ling Dan and herb-pair extract[J]. Journal of Separation Science, 2016,39(11).
11. Vinod Bhatt, Neeraj Kumar, Upendra Sharma, Bikram Singh. Comprehensive metabolic profiling of Zanthoxylum armatum and Zanthoxylum acanthopodium leaves, bark, flowers and fruits using ultra high performance liquid chromatography [J]. Separation Science Plus,2018,1(5).