Volume 11,Issue 2
Determination of Hydroxysafflor Yellow A Content in Mongolian Medicine Nidajindage by HPIC Method
Objective: To establish a high-performance liquid chromatography (HPLC) detection system for determining the content of hydroxysafflor yellow pigment A in Nidajindage. Methods: The HPLC scheme (using a 4.6mm inner diameter, 250mm long column, 5μm) was maintained at 35℃, and methanol acetonitrile (26:74) was used as the gradient elution mobile phase. Results: The detection wavelength was 403nm, the flow rate was 1 mL/min, and the injection volume was 20μL. The substance showed a good linear relationship in the range of 0.02μg/ml-0.1μg/ml, the regression equation is y=8000000x+36997, R=1,with an average recovery level of 92.4% and a relative standard deviation RSD of 0.4401%. Conclusion: This scheme is easy to operate, accurate in measurement, and has strong reproducibility, and can achieve quality control of Nidajindage.
[1] Yin W, Ba T, 2010, Determination of Hydroxyl Safflor Yellow A in Deligun-8 Powder by HPLC. Journal of Chinese Ethnic Medicine and Pharmacy, 16(12): 55-56.
[2] Li YY, Gao G, Xu H, 2010, Determination of Hydroxyl Safflor Yellow A in Chongde Ri-15 Pill. Medical Information (Mid-Monthly), 5(10): 2982-2983.
[3] Liang Y, Pei J, Wan DG, 2010, Determination of the main components in safflower yellow pigment. Medicinal Plants and Natural Products Research Conference of the Botanical Society of China Plant Medicine Professional Committee. Proceedings of the Ninth National Symposium on Medicinal Plants and Plant Medicine. Key Laboratory of Chinese Medicinal Materials Standardization, Ministry of Education, Chengdu University of Traditional Chinese Medicine: 305-307.
[4] Wang XP, Liu F, Ma CD, et al., 2010, Improvement of the determination method for hydroxysafflor yellow A content in safflower. Journal of Shaanxi College of Traditional Chinese Medicine, 33(04): 96-97.
[5] Chen J, Jin S, Suo LC, et al., 2010, Determination of Hydroxyl Safflor Yellow A in Sumu-4 Decoction by HPLC Method. Journal of Chinese Ethnic Medicine and Pharmacy, 16(03): 48-50.
[6] Jin YC, 2010, Comprehensive Utilization of Safflower and Study on New Extraction and Purification Process of Hydroxysafflor Yellow A. Zhejiang University.
[7] Wang GZ, Li Q, Song T, et al., 2009, Determination of puerarin and hydroxysafflor yellow A in Naodesheng Soft Capsules by HPLC method. Chinese Pharmacist, 12(06): 699-701.
[8] Zhu LP, 2008, Determination of Hydroxyl Safflor Yellow A in Fuyang Granules by HPLC Method. Heilongjiang Medical Journal, (05): 18-19.
[9] Meng H, Qige RT, Zhu YH, 2008, Determination of Hydroxyl Safflor Yellow A in Jiawei Tuximule-3 by HPLC Method. China Pharmaceutical Affairs, (02): 141-142.
[10] Wang ZY, Chen Y, Zou QW, et al., 2020, Study on the determination method of hydroxysafflor yellow A and ZnO content in Compound Mahong Powder. World Journal of Traditional Chinese Medicine, 15(13): 1903-1907.