Objective: To explore the clinical effect and safety of low-temperature plasma in the treatment of benign tumors in different parts of otorhinolaryngology, and to compare the difference between it and traditional surgical methods. Methods: This study is a retrospective study, including 60 patients with benign tumors of otorhinolaryngology from June 2023 to January 2025. According to the operation mode, they were divided into two groups: low temperature plasma group (n=30) and traditional operation group (n=30). The operation time, intraoperative blood loss, postoperative pain score, hospitalization time, complication rate and recurrence rate at one year after operation were compared between the two groups. Results: The intraoperative blood loss, operation time, postoperative pain score and hospitalization time in the low temperature plasma group were significantly better than those in the traditional operation group (P<0.05). There were no serious complications in the two groups, and there was no statistical difference in the recurrence rate one year after operation (P>0.05). Conclusion: Low-temperature plasma therapy is safe and effective in the treatment of benign tumors of otorhinolaryngology, and it has the advantage of minimally invasive, especially for tumors with rich blood supply and lesions in deep anatomical regions.