Objective: To explore the diagnostic efficacy and stability of a rapid diagnostic kit for snake venom. Methods: By preparing anti-snake venom immunoglobulin G (IgG), screening for species-specific IgG against snake venom, obtaining biotinylated specific IgG, fabricating detection enzyme-linked strips, and rapidly identifying snakebites, the preparation of a rapid diagnostic kit was completed. Eighty standard samples of snake venom from three primary venomous snake species (Agkistrodon halys, Trimeresurus stejnegeri, and Trimeresurus albolabris) in Renshou County were collected for preliminary experimental validation to verify the diagnostic performance of the rapid diagnostic kit. The sensitivity and specificity of the kit were calculated through clinical sample testing. The receiver operating characteristic (ROC) curve was plotted, and the area under the curve (AUC) was calculated to evaluate the overall diagnostic performance of the kit. Some of the detection enzyme-linked strips were stored under different storage conditions for a certain period, and they were taken out for testing at regular intervals to evaluate the stability of the kit under various storage conditions. Results: The area under the curve (AUC) for the rapid diagnostic kit in detecting Agkistrodon halys venom was 0.736 (95% confidence interval [CI]: 0.633 to 0.823), with a sensitivity of 84.21% and a specificity of 88.04%. For the detection of Trimeresurus mucrosquamatus venom, the AUC was 0.840 (95% CI: 0.746 to 0.926), with a sensitivity of 85.56% and a specificity of 92.11%. In the case of Trimeresurus stejnegeri venom, the AUC was 0.696 (95% CI: 0.576 to 0.776), with a sensitivity of 88.84% and a specificity of 93.15%. After storing the enzyme-linked immunosorbent assay strips at different temperatures (-20℃, 4℃, room temperature) for varying durations (1 month, 3 months, 6 months), the sensitivity and specificity remained unchanged. Conclusion: The rapid diagnostic kit for snake venom demonstrates significant effectiveness in snake venom diagnosis, exhibiting high sensitivity and specificity, along with strong stability, making it worthy of promotion and application.