Volume 11,Issue 1
Anti-Aging Effects of the Herbal Decoction Si Jun Zi Tang on Spleen Metabolites in Aging Mice: A Metabolomics Study
Objective: In this study, metabolomics analysis was conducted to assess changes in spleen metabolite profiles and pathways, aiming to identify key molecules and biochemical mechanisms that may contribute to its anti-aging effects. Methods: Thirty 3-month-old mice were divided into a young control group (n=10). The remaining 20 mice were aged to 6 months and then randomly assigned to a natural aging model group (n=10) or a Si Jun Zi Tang treatment group (n=10). Spleen tissues were analyzed using ultra-high-performance liquid chromatography coupled with high-definition quadrupole time-of-flight mass spectrometry. Data were analyzed with multivariate statistical analysis. Results: The spleen metabolic profiles differed significantly among the three groups. Multivariate analysis revealed 20 signature metabolites that were significantly altered in naturally aged mice compared to young controls. Of these, 12 were altered following Si Jun Zi Tang treatment. These signature metabolites were enriched in four key metabolic pathways. The treatment demonstrated a beneficial impact on these age-associated metabolites and pathways. Conclusion: In conclusion, Si Jun Zi Tang may mitigate age-related metabolic alterations in the spleen, thereby shedding light on the mechanisms underlying its anti-aging effects.
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